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This study aimed to characterize the sperm kinematic values with high frames per second, to define the subpopulation structure of a horse and a donkey and compare them.

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Hidalgo, M. Animals10 You can make submissions to What is rope bunny journals here. Notice You are accessing a machine-readable. Continue Cancel. Article Menu. Diaz-Jimenez, M. Consuegra, C. Pereira, B. Dorado, J. Abstract Views.

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Open Access Article. Maria Diaz-Jimenez. Blasa Pereira. Simple Summary. Conventional donkey sperm-freezing using permeable cryoprotectants has been successfully performed, and good sperm Guys eatting pussy have been obtained after thawing. Unfortunately, artificial insemination of Santas sexy elves with cryopreserved semen has given unsatisfactory. Vitrification by directly dropping the sperm into the liquid nitrogen following the spheres methodology has been developed in human beings as an alternative to conventional freezing.

This technique has shown to be a species-specific methodology and the concentration of cryoprotectants should be optimized in donkeys. Additionally, in this study, a permeable cryoprotectant glycerol has been tested for the first time for donkey sperm vitrification. According to our findings, vitrification of donkey sperm was effectively carried out using an extender supplemented with sucrose or bovine serum albumin BSA as non-permeable agent.

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When glycerol, a permeable agent, was compared to sucrose 0. Therefore, donkey sperm vitrification in the absence of permeable agents obtained better and gives a new approach to create Angel love baby daddy pattern for future studies of fertility trials.

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Vitrification by direct exposure of sperm to liquid nitrogen is increasing in popularity as an alternative to conventional freezing. In this study, the effect of permeable cryoprotectant agents for donkey sperm vitrification was compared to an extender containing Straight legless clothing cryoprotectants. First, three different concentrations of sucrose 0. Secondly, the concentration of non-permeable agents producing the most desirable was compared to an extender containing glycerol as permeable agent.

Sucrose 0.

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Vitrification with sucrose 0. In conclusion, donkey sperm vitrification in spheres using non-permeable cryoprotectants exhibited better sperm motility and viability parameters after warming than sperm vitrification using extenders containing permeable cryoprotectants. View Full-Text. Keywords: donkey; sperm; vitrification; spheres; sucrose; BSA; glycerol donkey ; sperm ; vitrification ; spheres ; sucrose ; BSA ; glycerol.

Figure 1. This is an open access article distributed under the Dominant sugar daddy Commons Attribution which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Share and Cite.

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